Fluorescence-polarization spectrum and electronic-energy transfer in proteins

Fluorescence-polarization spectrum and electronic-energy transfer in proteins.

The polarization of fluorescence and energy transfer in grana.

The results of several experiments suggest that t,he first step in green plant photosynt,hesis is a cooperative act carried out by a few hundred chlorophyll molecules. By using very short flashes of light, spaced far apart so as not to interfere with each other, Emerson and Arnold (1) found that the maximum number of carbon dioxide molecules reduced per flash was some 2500 times less 6han the n...

On the Quantitative Analysis of Electronic Energy Transfer/migration in Proteins Studied by Fluorescence Spectroscopy

Two recently developed theories of electronic energy transfer/migration were for the first time applied to real protein systems for extracting molecular distances. The partial donor-donor energy migration (PDDEM) is an extension to the previously developed donor-donor energy migration (DDEM, F Bergström et al PNAS 96, 1999, 12477) which allows using chemically identical but photophysically diff...

Discovery of coumarin derivatives as fluorescence acceptors for intrinsic fluorescence resonance energy transfer of proteins.

Coumarin analogues were synthezised and evaluated as acceptors for the intrinsic fluorescence resonance energy transfer (iFRET) of tryptophan residues in target proteins. The fluorescence properties such as quantum yields, iFRET efficiencies, and Förster distances of the prepared coumarin analogs were determined in a model system, by their conjugation to biotin, utilizing streptavidin (SAV) as ...

[13] Fluorescence Resonance Energy Transfer

of the extent of binding. This effect (known informally as "Chip dip") is illustrated in Figs. 6 and 7 which concern the ethidium bromide/tRNA system. 41 In this case the free ethidium bromide has a lifetime of 1.86 nsec and a rotational relaxation time of 0.54 nsec. On binding the PhetRNA Phe the lifetime increases to 26 nsec and the rotational relaxation increases to 136 nsec. A single rotati...